Measuring Ethylene on Perkin-Elmer GC

Measuring Ethylene on Perkin-Elmer GC
11-3-97 TSA

1. Detector A (top) is for whole cell assays and detector B is for enzyme assays.

2. Turn D to "column heater", left light is on. Push start ---> B light on.

3. Set "detector air" at 20 PSI.

4. "Detector A hydrogen" up, adjust hydrogen to 20 PSI.

5. "Detector A hydrogen" down, "detector polarity" at A+, push "ignite" and lift "detector A hydrogen" at the same time. Pop sound signifies that the GC has ignited.

6. G at 1024X, H at X1K. Warm up for 20 minutes.

7. Zero chart recorder, put G and H to 1X, then use E and F to zero.

8. Flush needle with N2, inject with 0.1 ml of sample.

9. CH4 comes out with a shadow first, then ethylene (C2H4). Acetylene (C2H6) has a huge peak about 20 minutes later; therefore, inject as many samples as possible within that time.

10. Turn off:
	"detector A hydrogen"	down
	hydrogen	off
	air	off
	D	off
	chart recorder	off